	   SnapGene      GTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATAGAAGACACCGGGACCGATCCAGCCTCCGGACTCTAGAGCTAGCGTTTAAACTTAAGCTTGGTACCGAGCTCGGATCCTAATACTTTCAGGACGATGACAAGAATGGCTTATTCAGAAGAGCATAAAGGTATGCCCTGTGGTTTCATCCGCCAGAATTCCGGCAACTCCATTTCCTTGGACTTTGAGCCCAGTATAGAGTACCAGTTTGTGGAGCGGTTGGAAGAGCGCTACAAATGTGCCTTCTGCCACTCGGTGCTTCACAACCCCCACCAGACAGGATGTGGGCACCGCTTCTGCCAGCACTGCATCCTGTCCCTGAGAGAATTAAACACAGTGCCAATCTGCCCTGTAGATAAAGAGGTCATCAAATCTCAGGAGGACTACAAGGACGTAAGAACAACTGAATTCTGCAGATATCCAGCACAGTGGCGGCCGCTCGAGTCTAGAGGGCCCCTTAAGGGTTCGATCCCTACCGGTTAGTAATGAGTTTGATATCTCGACAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGGAAACGGGGGAGGCTAACTGAAACACGGAAGGAGACAATACCGGAAGGAACCCGCGCTATGACGGCAATAAAAAGACAGAATAAAACGCACGGGTGTTGGGTCGTTTGTTCATAAACGCGGGGTTCGGTCCCAGGGCTGGCACTCTGTCGATACCCCACCGAGACCCCATTGGGGCCAATACGCCCGCGTTTCTTCCTTTTCCCCACCCCACCCCCCAAGTTCGGGTGAAGGCCCAGGGCTCGCAGCCAACGTCGGGGCGGCAGGCCCTGCCATAGCAGATCTGCGCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGCATCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGGGGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCGACGGATCGGGAGATCTCCCGATCCCCTATGGTCGACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGCTTAGG  8%                                       #      *                                                       !                                 !               !         "   "            *      $   -      #      *            !                                                      2                                                                                                       	      	                              $            #                     #   (                                                                                 +   )          !   *                           !                     "            #                                 *   3            #                                                  +      
               *          "      %            3   &            $      #         '      .         Y áœ-ýþo£ö$%É¢	ó›^›=ª,à. á xâ b¼¼.gÿš	£	<z“ }«-*Õtß¬` Z ÷ }SZp×&O×		ö	¡ñœ>á†ô/  ÈNÍr�æ}¤ä÷Ê	¼ôP�œ_8îÀrÙ5´ t û c’„)U E	=¥-¬Öa¼ �ß/÷"&m	å	ü	D
{1Çõ'~8]ã+`ñ1u•­½ ÎãúÇ³ó\Ú›	¤	Rdj…¡-F—Ú!.éÀ!à-[ â y!½Îc,5½ÿh�[	,€Oú”Å4 ø ¬_®v$TØPU
	÷	?
¢Äò?´Z“º–‡ Œ MRî1Ï'RþM£½âø?l�Í ´Õ=‰~�B˜qXØ	0	³	‘ü�êX�´–âVõ0 [ f[v„	8éé`âiF$v¿#  ÉOV^qÜø>LõQ�ß‚’ª÷.1Þ±Á÷v·Ö3 ò¾Ôµ ]‡ºRÎiž7‚ûËí
\
½2�Õ{ˆ²\™è* œ "D«~å°
P
Ë
(ª²Íæ:C…Ñâõ`{í9ï
ñ„s'ÚÒí   “ Tas]� ³ç¥Þÿ	«	è
î
"™GÎéŸMXjæ6A d*pSæ
ì
 h¨0•¯²ö†×½ “ðò˜^¦ÀÉ­ÿ¯=b u ü vi—VÕF	….I²g_}õ™ÅÆ tÖô(j…‘¾	þ
>!V(�Ø"MúE•ò:ŠÑ ³%Ž0#Û
`
	u|“2S%(ä,1PÉB2Av7–ç Ãøý	E
ÂÈ¤æþ9ºa¸ö!¾D KôEFØ	æ	 »4öš&±®Æ �=à'n	|$n=^ò÷  ¯È	e‚ÖÕ.G­…I� % ÏO�ô�)	/ÏÇÐ÷á 7 ä´Ç]Þ	‘	œ	¥	Ã	x†ÛaËÑê­  ŠÂûƒÛSXk¢ðAJ˜"vÁÌÔ".—ìþ,.?c\dl ¹ Å î AJûE•6‰6iÒ4-	?	-MiP�‚µ’¥çRX 1 ¶Ã¥ B`ÆÁÂ5n¸ö > ã zÚ"Ïd-Ë¾\	Ï
¸�ã•ÛÆÇ·’z   « p©¾‘šgõ£û=©­Ì ‘Áò! ù Ì`;‚Ë\ñ[ot"SV	Y	ã	ú	B
£AÅçõ›µ1”ïD¸ ­ÁÙe	¡üKC„�—ˆ #Yw€%PUQê	ì	ø	@
ßóú2[wÂ»H¶µ· ž¯ yäc–I@‰ïyíWIË S9	J]ýmŽÄƒÈ M � Õ N29’á4c¤¾ãa:�L È þ %•2qÐ
7(
c
DÆS²Â»ÿNù9@WÎ¸¤  3j‹¾[ïx'›‚ . ŠÞCXb™'RWB	lë˜bpi°n £0>Ç:Ã¹   C ~ ŽY´	ò	YW rŽ÷JWö›  µÖz¤Þ>~™‚_´1	oûEžóµyÙ—ŸE•É€! c w Ì crÓ©Ù	N	c	´
’áýTs‡ž0íûVdˆ¥†ãE1 /*×\Šó>žÉz†êa¿ÆUwÀ \ ¼ ñ D›þ†e®ºî	4
|
êoªê®½3u§× ígkI¿…	9¾­ãjœcü ê9lywH	
I
‰
¢´G%! ²ÓP‡_ÝeŽž±mù<JM8xÝàø/‘¨²õü‘¼  Ö	‘“«2¢³ø¸ô•ýó¿¶Ï  , | ÊWÜr½²cRß`¹nw×/Õ~"  “c-2?H^x€îö6[eƒ…ie¦Âãñ4˜F³<™ê6Í" ˆ»qLêü`

Y
]
ã
Žð)3]žfL5ÖÙï§ªµ>E]ô HSjŒ8
8
g
€
/ØeGR|£òL›Œš¤Óé ^*ãŸAÌ$
(
l
p
tæX‡kk‚�+’—³ekê–ý ÏÞW{OƒîŠ¾#{‰`Y8*  � ¡ùF†ùü3E¥À	Ì
ÎçD`†Òãî":*ð�àòsGot•MŠï7Ã ¡Ø•ìt/k «³;‰öÿÔO(y¬Þ$  ê±Ôü#Å�±æ)@�ì´Ò*AKðOÚrÌ	6{ÛÓˆ±î
- vöeá²H¬æ†¿	
Q
6Ñe·a|[gÄÔå…¨çóAhHpêÂÝÊ‹ª bé¨è­é
ï
šÙÜ˜½5k¸ÏêXÚ$k.7qB¡“# ! Ú .˜G§Ú¤×U¦ít¤	HgwÁ‹ NYÏÜS*Š¦zìK  ˜ YŽÈ ìðL¡´Åëó¬	ò
ø
#°pzÝ9Bš³X* j ” ¤ ² 7Ue=6l
t^õ‚Ë®ß 4z‚$	8	Ž
š
£
¯
¹
Ä
øý‹¸7nŠ�çÐ à q||ã#Ö	™	õ	ò~]+Ø4«³ó- 
Æ(+4T%ci+ E–7\Äè3¿ ,” e0>
�é³L_÷¾!  UhÝþ

O
[
ç
í
!'©1z�°LWiƒÕÿ‡±ñ˜çì sÕ'u�ñoå
]§U'×®±ñf| ²$Jó<E
_
Á%<0·È@6°ö $ ‰NÆ�ÊÎ¬Ë-Æ„>c ”µÂÑA™,	_Å
Á  "~ÿ[ã	X	•1JîƒìCGµHÊ L Ô ý 2iŠ½pw‘
b
Å8V`~‚9š�£Ç B b v }˜WÒñ	³
†/—UVhDq¯…�”ö=ÆDš šìjÖG	 
3
H
{
ˆ
y­³b¦ ÒÛ†’�;Gbwí5wh�²û.ó�”;} p)ÝV

#
7
X
k

"Wdj�*FQ–Øî¦©_ój‹éÒ u×éõ±2¾¿	ÿ
5?_)NÓÙïNŽænoxÜÝ i Çkì4j#8ŒÛR)-Ÿ /3{$Ü˜	}€èKVh2¾3ð+—ª²æë ´&�;Å@\€	T&;ÍÀeÇÊƒ !1ëÑÿ	
G
a

”Ä0¬CUB�8<¥ G oè1F½"
j
4v3±c€)7å-mQ¥wèžÜ .Ä?þ	!
F
i
3Ãçb/6:JUgÌd½Tÿ©F2 *º=>Jûüý·	¸	¹	Ë	Ì	12ÉITefžŸbc¹º»¼÷<=þ"Ž�œ¦§¨ EÕ � P+Jš!»;Äº	Í	Ê¥^r{ÿ 7>J�¿!)Ö 	âùùKiþh	¦€»~‚øc$#�% � Â ô GQÅ,K›”»	ç	!ÚË5¦U¯F—>?xôžÀ I ¿ Ý :G©U	¼_g|!‘²Úä¯ÇÞ"* S LõF¼Î	Ù	Ý	/
w
Ó
s \8ji×ù O "¨<ˆÅÉ÷ã›'¡KO† ¨ × lúLjðÓ	£2ó�é 4ú>o	r	È	}Â>å‘&ã … 
7ãUAØáÿi	”
©
%o@§R¦!ùdÊNø$_ ‚¸¬Ž(y	:
‚
¼�_¼€¸ƒ%¡N r ‘ õ RvªÆL B	É
ƒÏ/¨ÂÌ6VçÐTZt Ã Hn•Q2¼	fv¶"H§äG˜@]õüÿ  Ê -œÉÜïè	°¼AûJÁ ,c°4
&
e
n
á
×ÖÛ‰®(?yx†ºŸ‘ J g Ò Þ Ð°s*	V	†

hí�­’³ñ+˜ ƒ À m,9;Hg–	S`}-0ÊÈ"gâ°# & n p *kPžF½Y[ß ( õŽªèÄÐÑøÛåÈÎ	 4 ; ¶ ë >øBÈ¡Ô °	y‡ŒI]½k©äùú 8 F T _ `—¤öµC	Ú	0
x
Y”ë®9gá  å[½)ß’	�	¦	Ä	Ï	Ô
Ý
LÌt„; ì<Ø  MGÒ^‰	Þ	½
®ÜbÒ’Øjª ž‹æÆ!	lÊ>àUmwøœ¢ªÌµ-] P 'Â©YÌBZ¿Äm¬¹ä(™L€íMð  �# Ü	*
r
T£­K™#´PBÂÕ#P�˜â/dme‡¦ ›ÃüCåR„=‰*Îž
HÈñŸwÍ/©T^ÿ}@m  W Š Kß]Úû5Që.	N�P%ÞÓÝ»Y © Ó.KQƒÇ “}ÅôÎê º Æ ï B“ü7m7jwñ§	@	L	j%þžS * Ø 5F–Ÿê÷ŠMk¬0\Ô	Œê¶¤3¦E±èÀ ÄýYCaÃ#Âæ	6o¯’ 5˜¶1?¦¸p	É	~ÀÇ\pyœ€'„áä÷ 2 � l�ÀW?úï:	 ·ûns	Ã+¹ àBÌ}•j	¹&r‚pä�kØÒßbr“`	 ? .ÙÐ
Ç§"-Ë † ä {ƒ#äe¿p 	AÈeOùt Û8ÐVªâ…]	u	•
ª
)–¨SÜ	ú¸P�%SÊÐ{ ¡ ƒ¹¿ñÑŠ•hü>ª×Æ`’‡úÂç4ó §z¿§;
ƒ
	®Ò„uß&¢z qª­ªX’ð²ù½Í‚Z)‹N ¬ ó¹�)�›ÅÊöz	¤;½�¹O[ ö wÇMaÌ›åY¢	¡	 »Žð7ší<¿q… s ú Í‘bƒŒ²òþ[öD	Z	l	ä	û	C
„¤0NÆÙ,âð» «í<ÎAu—#TW	²	ÃWœèá>kŒ•hE¹ÌU•u$ ’ › S\†¹]!\pC�Ê
BF„Ð©Íèö-Ýu¶2ÔåÑ@[ iï–R½	ý
gŽHLD±^…˜®<‰Å €DÂ
Ú{#t$åý™OAÅæ  6 ®œ		(	�	Â	WwÕÔïIuÓöýb�# 0 = Ÿ ¸ Ä í @IoÙúD¨Êˆ53f>	Î
·¢<�¨Ú‘æm¶‘Qñ :��ÊÀðZxORâ	é	ë	ù	A
bæôšùûBvx
�´¶  $Z	68C±Á3I\üÃ·K Ë £Ý�&QVbb	àrDòúžm¢I9Â¸ .éHd­)‰í	én©=¡½…›¼2tû  ±b°ZÜ_d¸âôð—E˜»Ì âKz
'
f
o
â
.‰(×åK�‘z¢ñKdXü#  °àûE…ûGs¤åÐdˆ¶³!J)r@Lzî«‡ÉŠ©°	( -d5Ÿ¦Ù	£ÖïôT§s÷
Ø—¼üŠ¯·ÀAmŠ²p¥yÏ J’  ß T"Õ	ô	&‚K^þ, ±Ôtn+	Ö/,Å{ A K a Ó ™}�â	W	2
z
‡
–®„“´™É h ÑÚ	:~iÒîín�ò nç:zE—	¿€;‚±�Û Ž Oá-ø:<IhÃ¶	a~.5dË6;ISb#ý$ H N Á  “ T	Ü	Ò
TâE[–~i�hã „ Ö 3×Ç	Ò	“
¨
Q1¥ä ÉM q É +bu3ÛîP1
d
à
µãú\ÏY¹s ' o jlr8•	ERÃÉXZ : E ÿ =–£ÑÓˆ	¯	Ü
­‹H¼:Ÿfàãø &QŸ)~	G=Ç¾Tv³¸ Ož©ËAŽSLï¥  ) V ‰ 4’\éöv«[êéO$°ÝÍÒÜ € kŒ¿—0·m[*®ù
ƒà ‚(qœsÉÏ ¦ðy‰�øÅÑYtÞùæ3 …¸ìa‹šäX¡C	 	±	Ÿ�,ìÜ¾=jgË?º / 5 < · ì ?ØùC§h›É	=	�	zî€°tÒ;Äåð 
€¿5Yc(aa	Bm<C™ �l¡	1ú³ 
 ßD„¢ÕóSr-ˆYµ¶ J^c·q¡±o—¤ªÈÎû  ` ˜|�Ôá	ó	1
y
ì G àæ÷yÖÿDS	µ	Æ	Ñ	Û	Z•5:H}h�üœÚ 9 D atO”	Û
ß
GQ·�ÊRKXâî÷ U  ˆ ¥¶èZéNZX¯sÝøp2r 	 ·×	¦gš¾ƒã`<	Ž	Ÿ	,Ÿ¶<œ Ñ
–Ê {ßçþNÓàèR	“	Å	Ð	Ú
Þ
MF4GQûqíÙ+  ¶¥æ*½2	3	�	ž	Õ
Ö
×
Ø
Ù
+MÔü…†‡ˆz{˜™š›Ø	3FŠpØÙ) æ }æ%?\%+·ÁÇ=µ¾‹‘ž³4	§	NpÕÍýu‰ôt|Ú8Ù¡‹v�Ú=   €Â~ÂÈsÔŒª5	¨	Oq¯tcŸÎò4î'We]¢‡!F d !@³&d	ÝvŠ‰&¦ÕÙgwÛ! £ÖHÓ,_p¶’ŸO	Š	ß	¾
“þÖs}ˆ‹à1“ 9ÚkŒ«‚* x Í ç ~ç&N]¸È>•™¹¹¿•š´Úd	µ
âUCÓþõuÈÎ}ÛüäÔ2> ø¬	+ØtÕôÇ‚	©	P°Ë+ó{�çï(X^"^ g�£‹óg?áÏÊ£GVx 0x]m?œŸGù‡ëíb«£À¶ÄÇ.U  ŸŒÃçJÃÉè�¢«"	6	rcuVdnx 5ÒÞfÍˆÁ ‡"&f¯Ïï	5
}
ëC[)¯SŠì�’Ú4hñ Q ½ ò EÃÿª†»w	-
u
Z~­ª<ÅMîŸØ)Ü ] e (œA'äe«ÍÀÞ&ªºåë§ÖRv¨	Ìÿ Ý´	p°Ånw‹UiÖšCü'
¾Nx! ¤×I°`q®‡à	õ
û
UÓL×Œ˜¿CJäk™ã�¬ƒˆ�§ �hÒl Á†	tá”ÐÛQ�d ÔL·À·“P	_	‹	ÿ:~‰2µÆ®rÖ$nÌ0enÒ î$¡-JÝ €	+
s
¿
”¤®š$õ!:QÃ�l'ýf} y :ËDO¹ºÛ—
¬
¶
Á
–ãòÿÉ‡ý xUß)~3 è èð'œ­ÄXS?ŠI	e	IÉÔ£v ~jµª[_A ý^æã/2>´:m–ºˆ›V¦¹DöÏ÷Î0H&Õ" Î ë…ÉÏzšßîxÀ+–µÏ

J
S
Š
Ÿ
8]Üé#ºå ?hn¬  X à»»,füRnÖõNÈ;Oyš’|©)ßÔsÞ_ ÍùÞ�Ù!Q,ùô&_0¼Z ‹ L³ÔQ^ˆ­Œu/	ƒ	û7Q³èýY¢¼’á## Q`Û
€Ÿª²6ú=K¯ìª	˜±Ìå9Þáô‘ì0	žðö&z½Ó óhÔ—ë
/¿ ”„È!ù”õþ9ôÄÐ ×	’Rmº3£¹~õ C  Á‚ŒÝ@¬`Ð–Ïë+Hk ª ¤ôLhŽâ¬´Ë¤Àÿ�ÆÀ·Wyõ ËXns!dÞ@ Hˆî0Á·V Ç ”18	&àºb€  - } yÝa^³¨A	M	nSkúaìoxØc‡ÿ¤ŸÈ/ » ð Cý…8kx¾ò¹�È¬¾ÅÖT  + { d�¼,l±1I]Õ	7d„¡¥§m²üé5Î G‹Né@_s�ï\†ej4´=šD£7•*    ”ÓdëøÄø.j…ŽœëÑþ@Z
fqÃËÓßä§ò5Fg”G‰éÁ6Â3 — £ ± Ù 6X<�—FèëKÄÊêò­3y�£¬#	7	�
™
¢
®
¸
Ã
ñ
Û÷fvWoy·ÙÜ6‰™ÎW‰ë 	Å'g{âý

=
N
Z
b�ñ*ŸD\y*6[ÃÔç°“ò # rˆ#IÁMÐD
^
ä
Ä$°ð
«¶?5õ h‰¼o|þZð	²
xT7Uíp‚ëaŒFC€¢# ÆÜU°Œëav
6
W
~
ì4^igM�P‹×Ú¨“(,Û^i|  0ê2Ð¼[°«l¤Æï¤*åê )I™ ¹úg	Ê	 
h
0]qz¥HS�}��›¥(Ô R ¾ Ü ó FÄ §‡9¨.
v
ÈÙf®=Nwó ÙÝ…ø § 6T·@«k�ïq	x	9
�
Á»?¢·Mœè ^â m©A%
m
È
uÎˆ§Á'»æ@ûSw þ f m ‚ Ñ )¯+f…
ç	ì,€¬fðÍ— 3 ^ µ ê _÷AZ( ŸB¼
K ƒ‘X“¨×©
ë;  Ž�ÁšBäåÍ 	)
q
�
¬ËYßlÃl«˜¨á]´|l ÞžÙP/¦	K	‹J$ÆD|Äý¿º µüX¿oxV"›î9 ©ß|”„ot	qŒj×ÑÞ,OaR ò¸¾Ð¦©W”ÄÉï±Ö:†(ŠZyM  š [�±Í@–ýZõ€k	ºEƒMØï™+;tápÓà‹ä”„ ž îC‡4	'	Á	Í
ü
VÔsÓ#;�Ù�Nålµ­aPŽÄ ¢9èG~¬Nˆ7á	èa¨qåÀñø„ùHušsŽ8+  ¥ØáJúîúFar£¦¯ö
Ï'‡»äû²¿Žl�áó–DKðJc‰¨¯ @ ÓÙm!}%•ÑÕ.+z
 ÿÂ’
§
Ñ
DPR <¢iÚíÒ0}	‡	®	¬´<Š uâÈ¨�žer �‘–/ˆul÷#)ÜÌ­ß „ë`4òq	`	AžX´í;µ+BÛ¯sfÍ²ï ‡ såxÕP¶
œÉ|Üo1* å |$fåý$M¶ÀÆ<â¼Š��²Òç;	Q	Œ	* ÓLs7Ð×2P‰úou×   ¢²Õo}”˜¸ÁÇ¸”™Br|Šßñ3ÇÍ&\%Ô fÓ w÷«f¢Iç¡ò�	f*b›FÑÝæìÃT" Ü%ã…Îv	,
t
¯}%©ThÕ
©;BRÄëû‘	Qž(Ëþ~ Ñ¯K­¶†À^	ô
ú
Ò—ÏÅôIqmË&Ñ|Œ% ï9¬ÊWâ.1³ÎÞí‡

R
–
«
À
7•¥¸\†èö"i¹ZÞ(g« ºÌP‹º© ®:—äú™ó6ë %¡»y/ràÒ Àò–Üê
¾l�¹_«¿þ�8*ÃBjô „` ¸ÝÇa†½3 z – ¢ ° W;EÒcê÷œ»éñ+°i„Œ
˜
¡
­
·
Â
ð
›ÚÐý?YØÛ	pÊlˆ“ˆûVCè5¢4”& " gÅHn{ÀÛT‹záu

<
M
V
±
hxwŒOZÓTê'+`‹B{¡ ¦ Û /é(§Ïîùf	
¯º>¤¡k¶vç£] l ´ Ð é ¨ö@™®Y@ž	„
œ
»
Ç
JÊÿ¦Â«à\§{ ñ�¨½“¥¨ØV“ÃÈ.¥J	IÕ9!ÐÝ…íQL$ ™ Z�8çí?«íMY¢ô6&	§ºú±¾÷:ƒ�àø´IO® ;€þ‡Ñ||	­	‘
¦
$C*Œq# r_¡wä3µñ;n|“—Æá»‰˜œÑ:	W³q{Þ%›[Ï1Ö «É®0Í„µÍìñó
ù
EÎÐëY' • ¯ V_ö›ð*¯ƒ·‹
 
9ùŒ^o¾…Žº¼ÑAi' k ¥ ³ Ï fõ?m¼’y’�ÂÇÎà-ø

L
U
›
°
º
Æ
þ¥¹8=éæP\ :7ìv†ÌÐ{ƒÌà5{—Ð%	9	{	�
¥
Žßê$p­. ­ ® 89ô>º»u�‘ÊËï‘œÁÆËÌÍ,¶÷

K
T
�
¤
Å
<‹Œ�ÝÞ‚ƒ„»è@o  -H  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                            ,                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   '                                           	                                                         	                                                                                
                              2                              	                                                                                       3   !         "                   r   P                     O                                                                                                                                                                       ,                                                                                                                                                                                                                                                                                                                                                                                               '               	                   	                            
          2          	                             3 !   "       r P       O   né  p  p  pK  to  xé  ~¡?	¥{“©	k Å{u`“ö;—¢ôyœÎÏî5{	Â
Ýý¡Oa·
]
E	ýÚ¢iØ	ÀL‘” U.Nì
�eeE	¬ü
g
 ” .U²Ê˜ÚrìðL¨´/ü?Ak†¤
g
š
é
ï
ç;RÝãX|«¸9e¹ünª.YTÄ3Â ¢·“ô¢À·¢·Â¢ô¢S©§´Sº
M
ZvòF	=À_ÞXAJ\rhÞeT
Ä%ŽS%ù;
qrþ�S‡øØPU)É"cyà¹ÿ”	Q!¹ î Aû6iRÂÉUwËZ˜ù½ŽîšîÎíõ?ˆc„Y	…�ú�`˜FÍèž@KE»Í	rÖs#Nf¹ î Aû•6iõµÔR’åx* ?Š? tôyc!yWyyWf9rF Ú	Y”g$ØÍry¼yWFútôjrä_ÆFìTõ³¿$
l
‡(ÆóW¹fm°
éJ7'H­’wõÁ3c¼îúÎrs&v£Øí
P
õ?]„ˆæâ0�+¯}©Ä(+˜ÿ’	$¨_Ø @œyzàá
L
Ç
ê
$9™¦:ß�ËŠ ßØò ¨Š¨­5êƒS.`˜
U
îo`˜è�+À“¨@Ç
¦  tôjÍryä¼c!_yWÿ?Â¨_@Ç
$¦¶ÅÑ%ŽS%” UØÚìõH¡¨®æüt‚­
$
Y
l
ŠDagƒ‡°´ên‹øNs£*Ï¨Ø @œÇ
¦$
l
‡?’úÚR¡—!ÀË!-->c «�å 	lEßŒja9œß4GûÜ÷:D—
¬
¶
Á
‡UtôO��s#+Nf
W
×¨ú£KKE&
n
). ÉUwè. Yy	H	Y	ÂÉyaWUw ËÒ&l¡°ZCLŸ×]~ Å{(®rÅÿ^hø* ýµŠ×B	¹¨­ê÷Œœ^�“ˆ5é
½7fJ'^�ÉUwåfx}©¯Ä(Þ�?¾©ÁòZt‚	ç"ö  ð…iŠ½š�W²	WUÒlúÚRj¡—!À!-[ÂûƒðvÌ.þ?l™ Z�?Yôzá
<
M
x:ZÓàíIü“˜^ÿ ùùE¥ê`Î3]†ãfL�àò•ÖÙ§ï] “ T½rs]��æv¤Þÿ	úæ5F$ºº9:ù™ ºrÑ¬Ø
	–‡°'
x½�vúF$ê`3]fLÖÙ§] ùùE¥Î†ã�àò•ï™	œ
ÊÂà\{	‚.Ú5ë�%Ü _bùéùEž¥±èêù<J`ï
šÎÙ38]†ÝàãÏ/XfÚL�àòõk•ÖÙ§ïB]¼¡“™ Z�º?ºYôzá
<
M
9:ù™x :ZÓàºrÑãÌ(
p
Xkk—³k¯ÖÕ­…�Ì•j	¹‚Òß“gn{±
wTê`‹B¡Çd-FšBäÍ�
¨]|%ÎRë‘~îü
�­Äá x ¼¼g“êyw
I
‰
Š K]ƒýY}.	päPÆ`6orî%Îü
�Rë‘­~ÄþZí‚ëFß|”qŒj×ÑÞaE•‰µ¥ç½Õ• ¯ Vö›ð*¯ƒ‹
 
oemMx‘¨ÚÏi]ÕF	\	Ûa•ÑöšÛ&²·zòLhŽ�´È¤¾ÅTW‡ø"ÏØÛû
PUÓ+Œó¯ý^äk)4¬�¡n|“—Æ˜q{Þ%[#Û|12–~ Õ 2Yeo¡Y¤ãWr½÷�L³%ŽS%É·À·“‰2Æ$eÒiÕF	²ÚÏ\	•Û·zeo¡½�]ÛaÑöš&_béž±èù<Jï
šÙ8ÝàÏ/XÚõkB¼¡“ßþZ|”qŒj×ÑÞí‚ëFa"ä˜@ÿd @&dÝ¦Õ»Y~ + { d�¼,l±1I]Õ	7d„¡¥§m²üé5Îz – ¢ ° W;EÒcê÷œ»éñ+°i„Œ
˜
¡
­
·
Â
ð
›ÚÐý?YØÛ	pÊlˆ“ˆûVCè5¢4”²ÓP‡_ÝeŽž±mù<JM8xÝàø/‘¨²õü‘¼ 3j‹¾[ïx'›‚u×éõ±2¾¿	ÿ
5?_)NÓÙïNŽænoxÜÝ “c-2?H^x€îö6[eƒ…ie¦Âãñ4˜F³<™ê6Í@ ÓÙm!}%•ÑÕ.+zŠÂûƒÛSXk¢ðAJ˜"vÁÌÔ".—ìþ,.?c\dl  “ Tas]� ³ç¥Þÿ	«	è
î
"™GÎéŸMXjæ6AºÌP‹º© ®:—äú™ó6ë %¡»y/ràÒm©A%
m
È
uÎˆ§Á'»æ@ûSw þ  i “ œ TuÇ×éõkas"]±2D� «³ç~¥Þåìÿ°¾	«	¿	
P
Ë
è
î
ÿ
"(5?™ª²Íæ:CG_…Ñâõ4`j{Îéí)9ï
NÓÙï#8NŽŸñMXj„ænsŒÛ'RoÚæ)-6xÒÜAŸÝí' f m o q ‚ É Ñ )+bmu©jl¯+3rÛî8PA1f•	
%
d
m
…
È
à
EuµÎçˆ§Á	ã'R»Ãæì,@€ÉúXZ\¬ÏûSYfw¹ðÍ s—þº Æ ï B“ü7m7jwñ§	@	L	j%þžSâKz
'
f
o
â
.‰(×åK�‘z¢ñKdXüS LõF¼Î	Ù	Ý	/
w
Ó
s \8ji×ù«É®0Í„µÍìñó
ù
EÎÐëY'd*pSæ
ì
 h¨0•¯²ö†×½G àæ÷yÖÿDS	µ	Æ	Ñ	Û	Z•5:H}h�üœÚº Æ ï Bq“üeuª×éõ07­mª±27Xjw’ñ§¾ð	@	L	¿	>
ÿ
5?�_j²ù%)½éNÍÓÙïN‚Ž³æL_n÷Zoþ)‹xžÜNS¾Ýg�£‹óg?áÏÊ£GVx  ,     T           )ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßñ½>ð{‘92¥zS Ÿ°o–z´aÔX:kEeBcø‚­¼ôZð×½«è¡?I‘'Ò¢Â�DÎub>¸÷°Ž·_ýôt€ß:ãÓxÛâþ•Ý#Öãø‘•¶Õ»A�AÅ®4fôÝ ÃÎÄ¹?é@¬oçÂ’XJ†nÇ[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢mŽhëò`êÚ®ûè™[à£‹º˜A(ƒò‹C\ƒ ˆÞ"£‡5ÿÞ
 Ú=÷âÓ¾¯êÎ¯þþh÷¶Ï>>�Î8 ì8 7‡Zò¢ÀáËêö;>"�~¸Ì/®9ËúÃû[ÓëëóŽ<;Œ>ëÌºÏ¬þÑÄ°nþ <ûüøÀ9xWpPGYðP_Ìæ5•QÑe\Ü ,¨­•Ì°¿@àÞâÆßÿhÖsó*ëê¿ß÷ÿÔ¬Pý³O\°4?�dÑ¸Í8¹ &ÿs?‰0Í¥3‚
s4È|çz=3ù3ó¾¬êëúú»=ÊÏ¯òÔîVÀ6þ¤
ñ©#©Ã~ÇýUz@†
Ü’Û?†G
«?¨r�/rº–Hè¯W�uÞ×ù»šê†¦P ¬>h)Ua8j]U©s–¸�b"Ò�"KýÿM˜Õ66íi-5ô"KýÿM˜Õ66íi-5öKóþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóî>á9¯E$„ê¨ù¨ÕS¿:&oj°nQl«6Q�£sB»ÃÎ€�æ°æuÑÖÃŽá�<ŠðâÓ4?
ê¢Ô|~/þ�Ó0æÈ8=Ç+èÍ8ŽÇ(·…0ƒŽhR<A»âÛ·èï�Romƒ†5Œ0ãg:ÃŸ�ÚÇßSÛ6ÄÄÜžÁ�O¢ÆÐa¸Q6ÐÌôñx¬ô;PÌØüpÄ²È`Æ>:<Y¶`óªàklxƒÃ€ˆ0|æÐvL çò£… Ã‡Î¾Èêp›<ïÆ³LÛ>¹³ë¡Ä®¸L¸³‡ÇÔÇ?_�mÄjÆ½óÏ®aP :Álªððàa¾ôÌ»c�+¬ýÿ^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bâgË¹9 Ém‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«µŽÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷áË¿þ`±îÒu¸îód¥=&ã¿ŽˆŠ,á$ŒÄÕjS�uC¡ñÖ�…ã¤   !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  !I<?xml version="1.0"?><Features nextValidID="20"><Feature recentID="0" name="circ_0007234" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="793-1069" color="#a6acb3" type="standard"/></Feature><Feature recentID="19" name="FL" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1070-1081" color="#00ccff" type="standard"/></Feature><Feature recentID="18" name="AG" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="781-792" color="#ccffff" type="standard"/></Feature><Feature recentID="17" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6015" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5224-6015" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6016-6084" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2923-3717" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1181-1769" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4465-5053" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="13" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2085-2513" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="12" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2527-2856" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2707-2842" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3893-4014" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6085-6189" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1835-1883" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="4111-4117" color="#ffffff" type="standard"/><Segment range="4118-4135" color="#ffffff" type="standard"/><Segment name="-35" range="4136-4141" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4156-4177" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4063-4079" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4087-4103" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="1651" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="1268" maxFusedRunOn="1268" detectionMode="exactProteinMatch"><Segment range="1652-1663" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  D<Notes>
<UUID>a09b3453-c320-41eb-9b21-d26433f3590d</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="6:26:46">2018.12.13</Created>
<LastModified UTC="23:52:29">2022.10.18</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  a<StrandColors><TopStrand><ColorRange range="0..1081" colors="black"/><ColorRange range="1082..1091" colors="red"/><ColorRange range="1092..6404" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..1081" colors="black"/><ColorRange range="1082..1091" colors="red"/><ColorRange range="1092..6404" colors="black"/></BottomStrand></StrandColors>  ,     Q  ù         ù)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßñ½>ð{‘92¥zS Ÿ°oaÔX:kEeBcø‚­¼ôZð×½«è¡?I‘'Ò¢Â�DÎub>¸÷°Ž·_ýôt€ß:ãÓxÛâþ•Ý#Öãø‘•¶Õ»A�AÅ®4fôÝ ÃÎÄ¹?é@¬oçÂ’XJ†nÇ[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢mŽhëò`êÚ®ûè™[à£‹º˜A(ƒò‹C\ƒ ˆÞ"£‡5ÿÞ
 Ú=÷âÓ¾¯êÎ¯þþh÷¶Ï>>�Î8 ì8 7‡Zò¢ÀáËêö;>"�~¸Ì/®9ËúÃû[ÓëëóŽ<;Œ>ëÌºÏ¬þÑÄ°nþ <ûüøÀ9xWpPGYðP_Ìæ5•QÑe\Ü ,¨­•Ì°¿@àÞâÆßÿhÖsó*ëê¿ß÷ÿÔ¬Pý³O\°4?�dÑ¸Í8¹ &ÿs?‰0Í¥3‚
s4È|çz=3ù3ó¾¬êëúú»=ÊÏ¯òÔîVÀ6þ¤
ñ©#©Ã~ÇýUz@†
Ü’Û?†G
«?¨r�/rº–Hè¯W�uÞ×ù»šê†¦P ¬>h)Ua8j]U©s–¸�b"Ò�"KýÿM˜Õ66íi-5ô"KýÿM˜Õ66íi-5öKóþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóî>á9¯E$„ê¨ù¨ÕS¿:&oj°nQl«6Q�£sB»ÃÎ€�æ°æuÑÖÃŽá�<ŠðâÓ4?
ê¢Ô|~/þ�Ó0æÈ8=Ç+èÍ8ŽÇ(·…0ƒŽhR<A»âÛ·èï�Romƒ†5Œ0ãg:ÃŸ�ÚÇßSÛ6ÄÄÜžÁ�O¢ÆÐa¸Q6ÐÌôñx¬ô;PÌØüpÄ²È`Æ>:<Y¶`óªàklxƒÃ€ˆ0|æÐvL çò£… Ã‡Î¾Èêp›<ïÆ³LÛ>¹³ë¡Ä®¸L¸³‡ÇÔÇ?_�mÄjÆ½óÏ®aP :Álªððàa¾ôÌ»c�+¬ýÿ^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bâgË¹9 Ém‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«µŽÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷áË¿þ`±îÒu¸îód¥=&ã¿ŽˆŠ,á$ŒÄÕjS�uC¡ñÖ�…ã¤   !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  !I<?xml version="1.0"?><Features nextValidID="20"><Feature recentID="0" name="circ_0007234" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="781-1057" color="#a6acb3" type="standard"/></Feature><Feature recentID="19" name="FL" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1058-1069" color="#00ccff" type="standard"/></Feature><Feature recentID="18" name="AG" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="769-780" color="#ccffff" type="standard"/></Feature><Feature recentID="17" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6003" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5212-6003" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6004-6072" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2911-3705" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1169-1757" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4453-5041" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="13" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2073-2501" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="12" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2515-2844" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2695-2830" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3881-4002" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6073-6177" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1823-1871" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="4099-4105" color="#ffffff" type="standard"/><Segment range="4106-4123" color="#ffffff" type="standard"/><Segment name="-35" range="4124-4129" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4144-4165" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4051-4067" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4075-4091" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="1639" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="1256" maxFusedRunOn="1256" detectionMode="exactProteinMatch"><Segment range="1640-1651" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  D<Notes>
<UUID>a09b3453-c320-41eb-9b21-d26433f3590d</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="6:26:46">2018.12.13</Created>
<LastModified UTC="23:52:11">2022.10.18</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  a<StrandColors><TopStrand><ColorRange range="0..1069" colors="black"/><ColorRange range="1070..1079" colors="red"/><ColorRange range="1080..6392" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..1069" colors="black"/><ColorRange range="1070..1079" colors="red"/><ColorRange range="1080..6392" colors="black"/></BottomStrand></StrandColors>  *O     
  Ý         Ý)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßñ½>ð{‘92¥zS Ÿ°oy/H‚®4fôÝ ÃÎÄ¹?é@¬oçÂ’XJ†nÇ[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢mŽhëò`êÚ®ûè™[à£‹º˜A(ƒò‹C\ƒ ˆÞ"£‡5ÿÞ
 Ú=÷âÓ¾¯êÎ¯þþh÷¶Ï>>�Î8 ì8 7‡Zò¢ÀáËêö;>"�~¸Ì/®9ËúÃû[ÓëëóŽ<;Œ>ëÌºÏ¬þÑÄ°nþ <ûüøÀ9xWpPGYðP_Ìæ5•QÑe\Ü ,¨­•Ì°¿@àÞâÆßÿhÖsó*ëê¿ß÷ÿÔ¬Pý³O\°4?�dÑ¸Í8¹ &ÿs?‰0Í¥3‚
s4È|çz=3ù3ó¾¬êëúú»=ÊÏ¯òÔîVÀ6þ¤
ñ©#©Ã~ÇýUz@†
Ü’Û?†G
«?¨r�/rº–Hè¯W�uÞ×ù»šê†¦P ¬>h)Ua8j]U©s–¸�b"Ò�"KýÿM˜Õ66íi-5ô"KýÿM˜Õ66íi-5öKóþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóî>á9¯E$„ê¨ù¨ÕS¿:&oj°nQl«6Q�£sB»ÃÎ€�æ°æuÑÖÃŽá�<ŠðâÓ4?
ê¢Ô|~/þ�Ó0æÈ8=Ç+èÍ8ŽÇ(·…0ƒŽhR<A»âÛ·èï�Romƒ†5Œ0ãg:ÃŸ�ÚÇßSÛ6ÄÄÜžÁ�O¢ÆÐa¸Q6ÐÌôñx¬ô;PÌØüpÄ²È`Æ>:<Y¶`óªàklxƒÃ€ˆ0|æÐvL çò£… Ã‡Î¾Èêp›<ïÆ³LÛ>¹³ë¡Ä®¸L¸³‡ÇÔÇ?_�mÄjÆ½óÏ®aP :Álªððàa¾ôÌ»c�+¬ýÿ^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bâgË¹9 Ém‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«µŽÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷áË¿þ`±îÒu¸îód¥=&ã¿ŽˆŠ,á$ŒÄÕjS�uC¡ñÖ�…ã¤   !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Î<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5719" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4928-5719" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5720-5788" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2627-3421" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="885-1473" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4169-4757" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="13" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1789-2217" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="12" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2231-2560" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2411-2546" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3597-3718" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5789-5893" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1539-1587" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3815-3821" color="#ffffff" type="standard"/><Segment range="3822-3839" color="#ffffff" type="standard"/><Segment name="-35" range="3840-3845" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3860-3881" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3767-3783" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3791-3807" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="1355" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="972" maxFusedRunOn="972" detectionMode="exactProteinMatch"><Segment range="1356-1367" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="729-836" color="#ccffcc" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  D<Notes>
<UUID>a09b3453-c320-41eb-9b21-d26433f3590d</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="6:26:46">2018.12.13</Created>
<LastModified UTC="23:50:13">2022.10.18</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y<StrandColors><TopStrand><ColorRange range="0..785" colors="black"/><ColorRange range="786..795" colors="red"/><ColorRange range="796..6108" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..785" colors="black"/><ColorRange range="786..795" colors="red"/><ColorRange range="796..6108" colors="black"/></BottomStrand></StrandColors>  (Ì      ñ  {         {)ª3Ž³œFg2‡hi¡ä¦–ImiÀ-¤­“öf¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßñ½>ð{‘Åþ”
Æþ|)%„¨fìuµû�§U¨…F‡‚k¥æ(ïªs˜�œã©cê&ØæŽ¿&­ªï¾‰•¾
8»©„ˆ?(´5È2�â*8s_ýà £ß~-;êð¬êÿïæ�pÁ{lóãèüã�ÐÃŠxu¯ Š,N¾¯`ã³â(÷àkŒÀrúãœ¿¬?µ½0~¾¿0øãÃ¸Ãî¼Ë¬úÏíKïêÏ¿Ï�\ C—…wuŸüÎaÃYUUÍÀŠÊŠÙ\À+ôî,gýñö€=g?2®¾«ýÿýJÀ…Ð;4õË ÃCøöMŒÓ€;�o÷3ø“ÚS0Àˆ §3L‡Îw£Ó?“?;êÎ®¿¯«³Ü¬ðêÿ-Nåloê@¯’:œ7ìÕW¡¤`­É =³ødpª³ú‡(÷+©d�îŠõx]í›°¹®¨jeª jÃæ‚•V†¥ÕZ—1ikˆ"-) T¿ÐïôÐÑI�ScnÖ’Ó_B T¿ÐïôÐÑI�ScnÖ’Ó_d¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ãîšôRHAª��ê�U;ð¢fö«áµÐaÊ³a¡u74+¼<èkaà7]l8îóÈ¯-3@3ð®ª-GÇâð�áaxÐq3lƒƒÜp2¾ŒÓˆìr‹xS@x8æ€Å#À4¾-»~Žøñ&öØ8cXÃ6s¡¼9øý¬}õ1]³lLMÉìðº,m�Åm ìÏOŠÏCµÍ�ÇA»,‡öcã£Å›`�V:®¶Çˆ<8ƒÎmdÊ!š8Q:1`8|ëìŽ§	³Îük4Í³ë›>ºJë„À{€+1X|}LsõøöÜF¬kß<úæ
¬Ê¯ÏïLÀ¶8ºÏßõè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.&|±û“‘:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»XíÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~±Á°»ñ¿æí'[Žá�6JSÒn;øèˆ BÎHÌMV¥9×PÔ:hØQn:  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="17"><Feature recentID="16" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="1257" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="874" maxFusedRunOn="874" detectionMode="exactProteinMatch"><Segment range="1258-1269" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="15" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3693-3709" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3669-3685" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3762-3783" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3717-3723" color="#ffffff" type="standard"/><Segment range="3724-3741" color="#ffffff" type="standard"/><Segment name="-35" range="3742-3747" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1441-1489" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5691-5795" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3499-3620" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2313-2448" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2133-2462" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1691-2119" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4071-4659" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="2" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="787-1375" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2529-3323" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5621" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4830-5621" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5622-5690" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>5ac5fe10-fa1c-4df9-b5c3-ac25e142db8b</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="6:26:46">2018.12.13</Created>
<LastModified UTC="7:53:34">2022.1.5</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..6010" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..6010" colors="black"/></BottomStrand></StrandColors>  
”ý7zXZ  æÖ´F !   t/å£àw—
T] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷ÀnÉ;_x„¾XzAF¢•Ù>`FøŽIeÚ£¨™#ÍÝ#¹ãŠ¹W*´;p¯ù£‚™x¡$Ä"ÂQÙÐƒ»ÝÆo}²Ž $§¦»ä±­b�ÊåzIë^.£íÅ�Æ©›©Ðºþ;Š7,Q×Bÿ`÷|#Û™¼T×Fü•œpE�"Ãô³Ívÿ�†Þ)ó¦ÿr	Ò*²»	 *0x6°”þ•âƒš²ªL›ï¶:'Ö4*ÙUÝÄÓ�h­”27SÐ®Ä~47ÔB^Éz¾Ù�H|#a©M�Î®‘u:€kNPÐðUžƒ[;k¢ãŠ^p ÷y�‡î×#MVÄb+ïýpXß{±Šc­,ìadv=]ù?Ê5¿„up˜Íº¿7Q•Ñú"“ú@MSôŸ«ò}¥·äÿŸÑÙg‡¿ç´æXtâáYÂzáõ·iØx!âÃé$ÌµIå’—Nèø�öáO3Ê)¶È{~7êF‡`—FBü1=E}
9r<×A~NÅŸ8¥1ëòù:ª�*ëR‹¸¬Ä5ÒøxônÀ®êÒµÊÕÚ‹:]=ÿ±}3¸4&¼|X»òå´XZ"glù{Cì?]G_…×‹”s€4ßÓ“ÕŠ‡ËN|{¤¨³WZ0ºÚfÖ(ãçZ'ßyÈµ�h(®Rw=ˆo(ßXÀòœ^ ¼Ì+%Þœ¸°EÕ/®…0ÖDCó7¼L¢ÂÇÆHaæ]Ä{Tßº&y&2P¾†ð¬™n‰8s–$0C„ìgqþwÎAƒ¸!Þc}šÃ•±x/Ëqj–òÐ(s^_Ó68³ƒIHÒ¿k1[éÛ<aàkù\m�e.jüyµ01�J:�Ñ{ií®JDg‰sÇûFÕOl÷ãî LSìe“…ÌŠsÚ9dÜÖ¨±œ9šúìR‰v_m²Ü¶­›‚ˆë°Üx?Ü¤ùpH¢‘N¹XJïú‹âèq@…Ð?©;`ÚŠÀ5ú_èÒñÙQsD.9¢´Å&xÅ`e¶÷ç!ò©Ëµ/×'þGšÌ��ãâsÒù¸y‹ÏOÀK€8žÓãQ}vÒ¢åM5•ÿ¨ÿÿ*ïÓ]ˆW®M=5ª¶)Ô€×W9ÀE”m€ÎuÃBÌŒÙv—-¹K0bîfúd²î£ÌùÊšD—$Î#¬¥F˜G±šh„yÀNÞZ†\«µR˜·Ø»à¶ç€¬“Ÿ¤ÝÚ>“#e}IØ³Øþ6VÞG¨ÞÜ½ªwp•xµF¤S�×O¬Ñ#�ì;áL½c±OTR?‚¤ë¬L·í"5a¿ÄîTº´IUÊ’pä6É�GÛ² !%„¼^S(ª4¿|ÝWû�”%ØÈ9¼.#ô—Ö_l Û¼Åxt2�{b¬qmóbè&î¤šf)—žP`€ýKnÅÿ<…“Â-•Ãf6½Ó…ä£Í¼T7¤ò§Hh¨°ôýxÎ–JuÔÞ×Ù¦ Á
3’Ž¶Qƒšá˜¬	+H­½Wh÷l¢ì·r©™“W5°…Å)Bš"3‘àV¦€gô\‰Î~Š�ë/ä ·qŸøRÐ °ê˜›Ùw¢^o+*´#ˆ(bü]ŸÃdh3b!xýA9\6ÇoõÇiWôâ	\ôëÚˆ¤ l¾3"D°¿výeò&‰­§óó^œ²+Ö(›ËÆoÄQžôuÃšÁà‡Š8ˆi�)±Û?WÅH£µ˜XL’ƒÇ`ä$òhœ‘+þCC$&*¿·—œîk¢2¶Œ*,êìþYÉ$½6Ö  ŸÍÚ)Ð¾¬«ðšûåíèŠ¯Šò�Ê�@9Ù‹¡uU÷@÷0  aJÿØ Ç¯á�`ëèN·,Œ¿fkªÆx!½‡;H¦¹úÁ,9FÝê\’TÛAº�‰lî”$ðMV¨^–M„;½’ê§ºÖju0~ÄàBcÒ1°?@3o&H�gÕ�0}Œ¤‚mØœ¶hëèAñºHÑ)=±Œ²i×R3†¶·iþHiüè4K¶è¹ýO�‹`^9h–¾¨åpÕr�Ët6Ð˜É¦È°‚»¿hðù…ø4°ivj½èn,7t$¬Ñ€;Þ!Q•.GMPÝ·‘º¶q#@Ò‰àÅ§8m&Î”žEK´ytçÊ]TÏÂøõ!â$&¶	x•HãlI‘‘E2®	òÆJXÞT
]?½Úx·×Î	üR�Úu1ýR~ÁVÔL¼Ÿ2™ý—EÌaÂ%ƒ-XO˜K*tH$r£! 5ÎkÛM3òvÔ\x÷b(½cJ´—(ÄŠyÀ#L†™b*]Ó¢Íjù §2¿;OA¸ÿ`B%† ‡9×Àòg£{Á‹%+"NOÏbžI~ŠeP½ž¾]&«TxG¼D l––¶s†_IÕ>ºÂÆztäŠ`6]”>…ªÀ	ñ;ðéÃÓÀMî3ê‹‚néòºÐ>ÂO"Ð’KëQ¶}ÂW�rÅ¯ø/Ýžª?LbÛ­ð4É÷ÛS#léÉgÕvúŠ}"„µyLò2ÿ )ÖH(£¦¯ä¶¯@;�)ú³©BÐä”¢)àeðj¾ dÓÖÃ¹E¿vÍ€WÛ1?ÔqÎÆIaóžªã=ü§KÓ·ížéLRMý¼´fr·Ïhkêz€ŸÜpå~ÐNë¡Œðüíè¦&tücßün¶B¨Véñkéž6pn�óƒŸ‹…aB9ŒDÇÜÏüÌ/:œ—–åŸÌìÄ¹�Is=Ì‡a%>Ä6T.Z{ÑÈÇÅÛJ´¹�4 §‚§QT2c`™w`zôåðæ�ÿJàö’ÞLÀïûRë¥J¨ÕäVVÅ	ºxCÅÌ­HìŒä¯z»ÚdEqÿiÐè«†ÁÚg¡Œ§ÈÑWïúÑx Ÿí}æ~Ö%BÙw†ÈŽD»*>ð½S!ž{5¯½�cHJÊÚÞsTi Áx¦ÃmÊ›#@éµxjvWr+±HlÁ½Ò«Ôb‡æW]Ð]´-úüÑ˜‚œ´ÐŒÀþübFR¿£ŒGqG0QñÔGúð"ðÙ­Ó"e ”vSÆ,^ì8Ï»Êe+ nchö0nda©Ö(j¾Dt�8¶îPkäEevQ7`çæéa‰kÞe$˜»ªkU7kfÉ"ÕF@_Ãª	¼ð‹‰y4\Åº‡dSf…åîª{Od5¬;~�¨AÂÐèl·+ƒ’Ç¯‘?N–~zþá©“ÐmQ‰Nc@u�;á/"j_�¹dÿÃ“‚áçypR”Ã9Š5‚Dß‡Ñ¾?'{\ö¹aün@C‡•]ÝçØP—vŠ÷ÔXÂ?¾£(!×�žGÞm~Œ	V<B¥`½@$ÝÆí?slK`rQ^jN�1t¸»Ó¦,OÙÈÖDQíV�õë6Lq.„z¯ÞVÝuÄìn   ü¿ž¢ó÷Ï ð˜ï “ö€±Ägû    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %v<?xml version="1.0"?><Features nextValidID="22"><Feature recentID="0" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="1663" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="1280" maxFusedRunOn="1280" detectionMode="exactProteinMatch"><Segment range="1664-1675" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IEGR"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4099-4115" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4075-4091" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4168-4189" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="4123-4129" color="#ffffff" type="standard"/><Segment range="4130-4147" color="#ffffff" type="standard"/><Segment name="-35" range="4148-4153" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1847-1895" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6097-6201" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3905-4026" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2719-2854" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2539-2868" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2097-2525" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4477-5065" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1193-1781" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2935-3729" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"/></Q></Feature><Feature recentID="16" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6027" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5236-6027" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6028-6096" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="17" name="AG" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="781-792" color="#ccffff" type="standard"/></Feature><Feature recentID="18" name="FL" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1070-1081" color="#00ccff" type="standard"/></Feature><Feature recentID="19" name="circ_0007234" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="793-1069" color="#a6acb3" type="standard"/></Feature><Feature recentID="20" name="çŽ¯åŒ–ä»‹å¯¼åº�åˆ—" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1082-1093" color="#a6acb3" type="standard"/></Feature><Feature recentID="21" name="çŽ¯åŒ–ä»‹å¯¼åº�åˆ—" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="769-780" color="#a6acb3" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ¨<Notes>
<UUID>cb84f836-d1b3-4862-a198-de0986ed0137</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pCMV-AG-circ_0007234-FL-Neo</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="6:26:46">2018.12.13</Created>
<LastModified UTC="2:57:31">2025.9.18</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      DO  d              E              ÿÿÿÿYô? P                                                                                                                                                              ©<StrandColors><TopStrand><ColorRange range="0..6416" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..6416" colors="black"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
